Developing A Protein Purification Protocol

Table of Contents

Developing A Protein Purification Protocol

Overview

Introduction to Chromatography

Hydrophobic Interaction

Column Information

FPLC System Components

FPLC System

Extraction of Protein from Cells

Centrifuge

Extraction by Detergent

Extraction by Freezing

Introduction to GFP

Introduction to GFP

Results

First Column Tested

Second Column Tested

Third Column Tested

Conclusions

Email: ssep@shodor.org

Home Page: http://ssep.bwfund.org

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